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The lowered exploratory and social behaviors, and blood hyperserotonemia observed in KI males are all Main phenotypes noticed in animal products of ASD (Silverman et al.
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This behavioral assay occurred in two trials. In the primary trial, Every single animal was paired that has a littermate with the dwelling cage of the subject animal. In the second trial, Every animal was paired having a novel mouse with which they had no preceding Speak to. Except for animal pairing criteria, both phases ended up executed using the exact methodology. Two mice have been put in an vacant, plastic, square (25 × twenty five cm) arena and allowed to examine or interact with no interference. This interaction was recorded by using an overhead camcorder and scored in serious-time by TopScan monitoring application (CleverSys). Just one mouse in Every paired demo was marked with a small dot of white-out solution streaked on its fur to aid the TopScan software package in differentiating The 2 mice during the demo.
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This behavioral assay transpired in pursuing two trials: nonsocial and social odor detection. During the nonsocial odor detection demo, a cotton swab was briefly dipped in a ten% condensed milk Answer and hung from a corner of the home cage of the subject mouse for two min. During this time, the mouse was free to explore and communicate with the contents of its cage without the need of interference. The length of time expended inside of 5 cm from the scented swab or immediately interacting Along with the scented cotton swab was recorded.
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